Journal:

Article Title: Expansion of a CD28-Intermediate Subset among CD8 T Cells in Patients with Infectious Mononucleosis

doi: 10.1128/JVI.76.13.6602-6608.2002

Figure Lengend Snippet: The CD28-int CD8 T cells have cytotoxic activity specific for autologous EBV-transformed B cells. (A) Separation of CD8 T cells from a patient with AIM into CD28-positive, -int, and -negative fractions. Flow cytometric analyses of CD8 and CD28 expressions showed that CD8 T cells could be fractionated into CD28-positive (a), -int (b), and -negative (c) CD8 subsets. Throughout the culture period the cells were incubated in the presence of IL-2 (100 U/ml) and IL-10 (100 U/ml) to prevent apoptosis (23). Typical results of three different AIM patients are shown. (B) Comparison of cytotoxic activities among CD8/CD28 subsets. Each CD8/CD28 subset was separately isolated by two cycles of electronic sorting. The three fractionated CD8 subsets (a, b, and c) were tested in triplicate for cytotoxicity against 51Cr-labeled autologous BLC with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations). (C) Inhibition of the cytotoxicity with Ab to HLA class I in CD28-int T cells. The fractionated CD28-int CD8 T cells (Fig. ​(Fig.4A,4A, section b) and radiolabeled autologous BLC were incubated with an anti-class I monoclonal Ab (line f), isotype-matched mouse IgG Ab (line e), or medium alone (line d). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated. (D) Cytotoxic functions against other targets. The fractionated CD28-int subsets (HLA-A11/A26 and B52/B56) were tested in triplicate for cytotoxicity against 51Cr-labeled allogeneic BLC (HLA-A2/A24 and B35/B46; line g) and K562 (line h) cells with various effector/target ratios (1). After 5 h of incubation, the radioactivity was counted and the percentage of specific lysis was calculated (means ± standard deviations).

Article Snippet: For blocking experiments, an anti-class I MAb (Clone W6/32; Cederlane, Hornby, Ontario, Canada) or isotype-matched mouse IgG antibodies were used at a final concentration of 50 μg/ml.

Techniques: Activity Assay, Transformation Assay, Incubation, Isolation, Labeling, Radioactivity, Lysis, Inhibition

Journal: EMBO Reports

Article Title: An hepatitis B and D virus infection model using human pluripotent stem cell-derived hepatocytes

doi: 10.1038/s44319-024-00236-0

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit anti-FoxA2 (1:400) was purchased from Cell Signaling, mouse anti-AFP (1:1000) from Sigma-Aldrich, mouse anti-ALB (1:1000) from Cedarlane, mouse anti-CD63 from Santa Cruz Biotechnology (1:400), rabbit anti-ADAR1 from Cell Signaling (1:1000), rabbit anti-LDLR from Abcam (1:200), rabbit anti-LAMP1 (1:200) and rabbit anti-EGFR (1:100) from Cell Signaling and rabbit anti-SCARB1 from Novus Biologicals (1:500).

Techniques: Recombinant, Immunofluorescence, Western Blot, Sequencing, Membrane, Knock-Out, Transfection, Reverse Transcription, SYBR Green Assay, Protease Inhibitor, Diagnostic Assay, Software, Microscopy, Imaging